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| Registros recuperados : 17 | |
| 1. |  | BARBOSA, E. A.; BONFIM JÚNIOR, M. F.; BLOCH JUNIOR, C.; ENGLER, G.; ROCHA, T. L.; ENGLER, J. de A. Imaging mass spectrometry of endogenous polypeptides and secondary metabolites from galls induced by root-knot nematodes in tomato roots. Molecular Plant-Microbe Interactions: MPMI, v. 31, n. 10, p. 1048-1059, 2018. Na publicação: Thales Rocha.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | VAN MONTAGU, M.; HOLSTERS, M.; ZAMBRYSKI, P.; HERNALSTEENS, J. P.; DEPICKER, A.; BEUCKELEER, M. DE; ENGLER, G.; LEMMERS, M.; WILLMITZER, L.; SCHELL, J. The interaction of Agrobacterium ti-plasmid DNA and plant cells. R. Soc. Lond., v.210, p.351-365, 1980.| Biblioteca(s): Embrapa Agrobiologia. |
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| 4. | | COELHO, R. R.; VIEIRA, P.; SOUZA JÚNIOR, J. D. A. de; MARTIN-JIMENEZ, C.; VEYLDER, L. de; CAZARETH, J.; ENGLER, G.; GROSSI-DE-SA, M. F.; ENGLER, J. de A. Exploiting cell cycle inhibitor genes of the KRP family to control root-knot nematode induced feeding sites in plants. Plant, Cell and Environment, v. 40, p. 1174-1188, 2017.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | CABRAL, D.; BANORA, M. Y.; ANTONINO, J. D.; RODIUC, N.; VIEIRA, P.; COELHO, R. R.; CHEVALIER, C.; EEKHOUT, T.; ENGLER, G.; DE VEYLDER, L.; GROSSI-DE-SA, M. F.; ENGLER, J. de A. The plant WEE1 kinase is involved in checkpoint control activation in nematode-induced galls. The New phytologist, v. 225, n. 1, p. 430-447, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | ANTONINO DE SOUZA JÚNIOR, J. D.; COELHO, R. R.; LOURENÇO, I. T.; FRAGOSO, R. R.; VIANA, A. A. B.; MACEDO, L. L. P.; SILVA, M. C. M.; CARNEIRO, R. M. D. G.; ENGLER, G.; ALMEIDA-ENGLER, J. de; GROSSI DE SA, M. F. Knocking-down Meloidogyne incognita proteases by plant-delivered dsrna has negative pleiotropic effect on nematode vigor. Journal of Nematology, v. 46, n. 2, p. 135, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | SOUZA JÚNIOR, J. D. A. de; COELHO, R. R.; LOURENÇO, I. T.; FRAGOSO, R. da R.; VIANA, A. A. B.; MACEDO, L. L. P. de; SILVA, M. C. M. da; CARNEIRO, R. M. D. G.; ENGLER, G.; ALMEIDA-ENGLER, J. de; GROSSI DE SÁ, M. F. Knocking-down Meloidogyne incognita proteases by plant-delivered dsRNA has negative pleiotropic effect on nematode vigor. Plos One, v. 8, n. 12, e85364, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | ANTONINO-DE-SOUZA JÚNIOR, J. D.; SOUZA, D. S. L.; GROSSI-DE-SÁ, M.; ROCHA, T. L.; FRAGOSO, R. da R.; BARBOSA, A. E. A. D.; OLIVEIRA, G. R.; NAKASU, E. Y. T.; SOUSA, B. A.; PIRES, N. F.; DUSI, D.; CARNEIRO, R. M. D. G.; ROMANO, E.; ALMEIDA-ENGLER, J. DE.; ENGLER, G.; GROSSI-DE-SA, M. F. Ectopic expression of a Meloidogyne incognita dorsal gland protein in tobacco accelerates the nematode's life cycle. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 2., 2009, Búzios, RJ. Programa e resumos... [Ribeirão Preto]: Sociedade Brasileira de Genética, 2009. p. 211| Biblioteca(s): Embrapa Cerrados. |
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| 9. | | SOUZA, D. S. L.; ROCHA, T. L.; SOUZA JUNIOR, J. D. A.; PINTO, E. R. de C.; BARBOSA, A. E. A. D.; OLIVEIRA, G. R.; FRAGOSO, R. R.; NAKASU, E. Y. T.; SOUSA, B. A.; PIRES, N. F.; DUSI, D. M. de A.; CARNEIRO, R. M. D. G.; ALMEIDA ENGLER, J.; ENGLER, G.; SA, M. F. G. de. Ectopic expression of a meloidogyne incognita dorsal gland protein in tobacco accelerates the nematode`s life cycle. Tropical Plant Pathology, Brasília, DF, v. 34, p. S285, 2009. Suplemento. Edição dos Resumos do XLII do Congresso Brasileiro de Fitopatologia, Rio de Janeiro, RJ, 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M.; ALBUQUERQUE, E. V. S.; GROSSI-DE-SA, M. F. Molecular cloning and characterization of an α-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei. Gene, v. 553, n. 1, p. 7-16, Dec. 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M. da; SA, M. F. G. de; FREIRE, E. V. S. A. Molecular cloning and characterization of an a-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei. Gene, v. 553, n. 1, p. 7-16, Dec. 2014.| Biblioteca(s): Embrapa Cerrados. |
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| 12. | | ALBUQUERQUE, E. V. S.; BEZERRA, C. A.; ROMERO, J. V.; VALENCIA, J. W. A.; VALENCIA-JIMÉNEZ, A.; PIMENTA, L. M.; BARBOSA, A. E. A. D.; SILVA, M. C. M.; MENEGUIM, A. M.; SÁ, M. E. L.; ENGLER, G.; ALMEIDA-ENGLER, J. de; FERNANDEZ, D.; GROSSI-DE-SA, M. F. Seed-specific stable expression of the alpha-AI1 inhibitor in coffee grains and the in vivo implications for the development of the coffee berry borer. Tropical Plant Biology, v. 8, p. 98-107, 2015.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | FIRMINO, A. A. P.; FONSECA, F. C. de A.; MACEDO, L. L. P. de; COELHO, R. R.; SOUZA JÚNIOR, J. D. A. de; TOGAWA, R. C.; SILVA JUNIOR, O. B. da; PAPPAS JÚNIOR, G. J.; SILVA, M. C. M. da; ENGLER, G.; GROSI DE SÁ, M. F. Transcriptome analysis in cotton Boll Weevil (Anthonomus grandis) and RNA interference in insect pests. Plos One, v. 8, n. 12, e85079, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | COELHO, R. R.; SOUZA JUNIOR, J. D. A. de; FIRMINO, A. A. P.; MACEDO, L. L. P. de; FONSECA, F. C. A.; TERRA, W. R.; ENGLER, G.; ENGLER, J. de A.; SILVA, M. C. M. da; GROSSI-DE-SA, M. F. Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females. Meta Gene, v. 9, p. 173-180, 2016.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | SOUZA, D. dos S. DE L. E; SOUZA JUNIOR, J. D. A. DE; GROSSI DE SÁ, M.; ROCHA, T. L.; FRAGOSO, R. da R.; BARBOSA, A. E. A. DE D.; OLIVEIRA, G. R. DE; NAKASU, E. Y. T.; SOUSA, B. A. DE; PIRES, N. F.; DUSI, D. M. de A.; CARNEIRO, R. M. D. G.; PINTO, E. R. de C.; ALMEIDA-ENGLER, J. DE; ENGLER, G.; MARTINS-DE-SÁ, C.; SA, M. F. G. de. Ectopic expression of a Meloidogyne incognita dorsal gland protein in tobacco accelerates the formation of the nematode feeding site. Plant Science, v. 180, p. 276-282, 2011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | SOUZA, D. DOS S. DE L. E; SOUZA JUNIOR, J. D. A. DE; GROSSI DE SÁ, M.; ROCHA, T. L.; FRAGOSO, R. da R.; BARBOSA, A. E. A. DE D.; OLIVEIRA, G. R. DE; NAKASU, E. Y. T.; SOUSA, B. A. DE; PIRES, N. F.; DUSI, D. M. de A.; CARNEIRO, R. M. D. G.; PINTO, E. R. de C.; ALMEIDA-ENGLER, J. DE; ENGLER, G.; MARTINS-DE-SÁ, C.; SA, M. F. G. de. Ectopic expression of a Meloidogyne incognita dorsal gland protein in tobacco accelerates the formation of the nematode feeding site. Plant Science, v. 180, p. 276-282, 2011.| Biblioteca(s): Embrapa Cerrados. |
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| 17. | | FRAGOSO, R. da R.; ARRAES, F. B. M.; LOURENCO, I. T.; MIRANDA, V. J.; BASSO, M. F.; FERREIRA, A. V. J.; VIANA, A. A. B.; LINS, C. B. J.; LINS, P. C.; MOURA, S. M.; BATISTA, J. A. N.; SILVA, M. C. M. da; ENGLER, G.; MORGANTE, C. V.; LISEI-DE-SA, M. E.; VASQUES, R. M.; ALMEIDA-ENGLER, J. de; SA, M. F. G. de. Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression. Planta, v. 256, n. 4, 2022. Na publicação: Rodrigo Rocha Fragoso; Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| Registros recuperados : 17 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
20/09/2022 |
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Data da última atualização: |
13/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FRAGOSO, R. da R.; ARRAES, F. B. M.; LOURENCO, I. T.; MIRANDA, V. J.; BASSO, M. F.; FERREIRA, A. V. J.; VIANA, A. A. B.; LINS, C. B. J.; LINS, P. C.; MOURA, S. M.; BATISTA, J. A. N.; SILVA, M. C. M. da; ENGLER, G.; MORGANTE, C. V.; LISEI-DE-SA, M. E.; VASQUES, R. M.; ALMEIDA-ENGLER, J. de; SA, M. F. G. de. |
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Afiliação: |
RODRIGO DA ROCHA FRAGOSO, CNPAE; FABRICIO BARBOSA MONTEIRO ARRAES; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; VÍVIAN JESUS MIRANDA; MARCOS FERNANDO BASSO; ANDRÉ VINICIUS JÚLIO FERREIRA; ANTÔNIO AMÉRICO BARBOSA VIANA; CAMILA BARROZO JESUS LINS; PHILIPPE CASTRO LINS; STÉFANIE MENEZES MOURA; JOÃO AGUIAR NOGUEIRA BATISTA, Federal University of Minas Gerais; MARIA CRISTINA MATTAR DA SILVA, Cenargen; GILBERT ENGLER, Université Côte d'Azur, France; CAROLINA VIANNA MORGANTE, CPATSA; MARIA EUGÊNIA LISEI-DE-SA, Minas Gerais Agricultural Research Company; RAQUEL MEDEIROS VASQUES; JANICE DE ALMEIDA-ENGLER, Université Côte d'Azur, France; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Planta, v. 256, n. 4, 2022. |
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DOI: |
https://doi.org/10.1007/s00425-022-03980-6 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Rodrigo Rocha Fragoso; Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. |
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Conteúdo: |
Soybean (Glycine max) is one of the most important agricultural commodities worldwide and a major protein and oil source. Herein, we identified the soybean ubiquitin-conjugating (E2) enzyme gene (GmUBC4; Glyma.18G216000), which is significantly upregulated in response to Anticarsia gemmatalis attack and Meloidogyne incognita-induced galls during plant parasitism by plant nematode. The GmUBC4 promoter sequence and its different modules were functionally characterized in silico and in planta using transgenic Arabidopsis thaliana and G. max lines. Its full-length transcriptional regulatory region (promoter and 5´-UTR sequences, named pUceS8.3 promoter) was able to drive higher levels of uidA (?-glucuronidase) gene expression in different tissues of transgenic A. thaliana lines compared to its three shortened modules and the p35SdAMV promoter. Notably, higher ?-glucuronidase (GUS) enzymatic activity was shown in M. incognita-induced giant cells when the full pUceS8.3 promoter drove the expression of this reporter gene. Furthermore, nematode-specific dsRNA molecules were successfully overexpressed under the control of the pUceS8.3 promoter in transgenic soybean lines. The RNAi gene construct used here was designed to post-transcriptionally downregulate the previously characterized pre-mRNA splicing factor genes from Heterodera glycines and M. incognita. A total of six transgenic soybean lines containing RNAi gene construct were selected for molecular characterization after infection with M. incognita pre-parasitic second-stage (ppJ2) nematodes. A strong reduction in the egg number produced by M. incognita after parasitism was observed in those transgenic soybean lines, ranging from 71 to 92% compared to wild-type control plants. The present data demonstrated that pUceS8.3 is a gene promoter capable of effectively driving dsRNA overexpression in nematode-induced giant cells of transgenic soybean lines and can be successfully applied as an important biotechnological asset to generate transgenic crops with improved resistance to root-knot nematodes as well as other pests. MenosSoybean (Glycine max) is one of the most important agricultural commodities worldwide and a major protein and oil source. Herein, we identified the soybean ubiquitin-conjugating (E2) enzyme gene (GmUBC4; Glyma.18G216000), which is significantly upregulated in response to Anticarsia gemmatalis attack and Meloidogyne incognita-induced galls during plant parasitism by plant nematode. The GmUBC4 promoter sequence and its different modules were functionally characterized in silico and in planta using transgenic Arabidopsis thaliana and G. max lines. Its full-length transcriptional regulatory region (promoter and 5´-UTR sequences, named pUceS8.3 promoter) was able to drive higher levels of uidA (?-glucuronidase) gene expression in different tissues of transgenic A. thaliana lines compared to its three shortened modules and the p35SdAMV promoter. Notably, higher ?-glucuronidase (GUS) enzymatic activity was shown in M. incognita-induced giant cells when the full pUceS8.3 promoter drove the expression of this reporter gene. Furthermore, nematode-specific dsRNA molecules were successfully overexpressed under the control of the pUceS8.3 promoter in transgenic soybean lines. The RNAi gene construct used here was designed to post-transcriptionally downregulate the previously characterized pre-mRNA splicing factor genes from Heterodera glycines and M. incognita. A total of six transgenic soybean lines containing RNAi gene construct were selected for molecular characterization after infect... Mostrar Tudo |
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Palavras-Chave: |
GmUBC4 promoter/gene; Ubiquitin-conjugating (E2) enzyme gene promoter. |
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Thesagro: |
Anticarsia Gemmatalis; Glycine Max; Meloidogyne Incognita; Soja. |
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Thesaurus NAL: |
RNA interference; Soybeans. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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Marc: |
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001 2148961
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100 1 $aFRAGOSO, R. da R.
245 $aFunctional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression.$h[electronic resource]
260 $c2022
500 $aNa publicação: Rodrigo Rocha Fragoso; Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.
520 $aSoybean (Glycine max) is one of the most important agricultural commodities worldwide and a major protein and oil source. Herein, we identified the soybean ubiquitin-conjugating (E2) enzyme gene (GmUBC4; Glyma.18G216000), which is significantly upregulated in response to Anticarsia gemmatalis attack and Meloidogyne incognita-induced galls during plant parasitism by plant nematode. The GmUBC4 promoter sequence and its different modules were functionally characterized in silico and in planta using transgenic Arabidopsis thaliana and G. max lines. Its full-length transcriptional regulatory region (promoter and 5´-UTR sequences, named pUceS8.3 promoter) was able to drive higher levels of uidA (?-glucuronidase) gene expression in different tissues of transgenic A. thaliana lines compared to its three shortened modules and the p35SdAMV promoter. Notably, higher ?-glucuronidase (GUS) enzymatic activity was shown in M. incognita-induced giant cells when the full pUceS8.3 promoter drove the expression of this reporter gene. Furthermore, nematode-specific dsRNA molecules were successfully overexpressed under the control of the pUceS8.3 promoter in transgenic soybean lines. The RNAi gene construct used here was designed to post-transcriptionally downregulate the previously characterized pre-mRNA splicing factor genes from Heterodera glycines and M. incognita. A total of six transgenic soybean lines containing RNAi gene construct were selected for molecular characterization after infection with M. incognita pre-parasitic second-stage (ppJ2) nematodes. A strong reduction in the egg number produced by M. incognita after parasitism was observed in those transgenic soybean lines, ranging from 71 to 92% compared to wild-type control plants. The present data demonstrated that pUceS8.3 is a gene promoter capable of effectively driving dsRNA overexpression in nematode-induced giant cells of transgenic soybean lines and can be successfully applied as an important biotechnological asset to generate transgenic crops with improved resistance to root-knot nematodes as well as other pests.
650 $aRNA interference
650 $aSoybeans
650 $aAnticarsia Gemmatalis
650 $aGlycine Max
650 $aMeloidogyne Incognita
650 $aSoja
653 $aGmUBC4 promoter/gene
653 $aUbiquitin-conjugating (E2) enzyme gene promoter
700 1 $aARRAES, F. B. M.
700 1 $aLOURENCO, I. T.
700 1 $aMIRANDA, V. J.
700 1 $aBASSO, M. F.
700 1 $aFERREIRA, A. V. J.
700 1 $aVIANA, A. A. B.
700 1 $aLINS, C. B. J.
700 1 $aLINS, P. C.
700 1 $aMOURA, S. M.
700 1 $aBATISTA, J. A. N.
700 1 $aSILVA, M. C. M. da
700 1 $aENGLER, G.
700 1 $aMORGANTE, C. V.
700 1 $aLISEI-DE-SA, M. E.
700 1 $aVASQUES, R. M.
700 1 $aALMEIDA-ENGLER, J. de
700 1 $aSA, M. F. G. de
773 $tPlanta$gv. 256, n. 4, 2022.
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